Publications by authors named "J Rossant"
Article Synopsis
- - Stem cell-based embryo models are essential for studying early human development, but their effectiveness depends on how closely they mimic real embryos at the molecular, cellular, and structural levels.
- - Researchers created a comprehensive reference dataset by integrating six existing datasets that track human development from the zygote stage to the gastrula stage, providing a benchmark for comparing human embryo models.
- - Using an advanced analysis method called stabilized Uniform Manifold Approximation and Projection, the new reference helps identify cell types in various embryo models, revealing potential misannotations without proper reference comparisons.
Physical processes ultimately shape tissue during development. Two emerging proposals are that cells migrate toward stiffer tissue (durotaxis) and that the extent of cell rearrangements reflects tissue phase, but it is unclear whether and how these concepts are related. Here, we identify fibronectin-dependent tissue stiffness as a control variable that underlies and unifies these phenomena in vivo.
View Article and Find Full Text PDFIn the rapidly moving field of stem cell and embryo research, research questions often sit at the intersection of scientific inquiry and ethical considerations. The International Society for Stem Cell Research (ISSCR) produces guidelines to help navigate decisions in this area. For Cell's 50th Anniversary Focus on Developmental Biology, scientific editor Sarah Geisler discussed the importance of the ISSCR guidelines on stem cell and embryo research for both the stem cell community and the broader public with Amander Clark, Robin Lovell-Badge, and Janet Rossant, who have been involved in the ongoing evolution of the guidelines.
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- MECP2 duplication syndrome (MDS) is a neurodevelopmental disorder linked to genetic duplications of the MECP2 gene and nearby genes like IRAK1.
- Existing mouse models for MDS often only express MECP2, limiting research potential.
- A new CRISPR/Cas9 tandem duplication mouse model called 'Mecp2 Dup' accurately mimics human MDS and shows significant neurobehavioral issues and immune response abnormalities, making it useful for studying the disorder and exploring therapies.
Understanding the processes and mechanisms underlying early human embryo development has become an increasingly active and important area of research. It has potential for insights into important clinical issues such as early pregnancy loss, origins of congenital anomalies and developmental origins of adult disease, as well as fundamental insights into human biology. Improved culture systems for preimplantation embryos, combined with the new tools of single cell genomics and live imaging, are providing new insights into the similarities and differences between human and mouse development.
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