Deaminase-mediated chromatin accessibility profiling with single-allele resolution.

Binding of transcription factors (TFs) at gene regulatory elements controls cellular epigenetic state and gene expression. Current genome-wide chromatin profiling approaches have inherently limited resolution, complicating assessment of TF occupancy and co-occupancy, especially at individual alleles. In this work, we introduce Accessible Chromatin by Cytosine Editing Site Sequencing with ATAC-seq (ACCESS-ATAC), which harnesses a double-stranded DNA cytosine deaminase (Ddd) enzyme to stencil TF binding locations within accessible chromatin regions.

Impact of blur on 3D laser imaging: Monte-Carlo modelling for underwater applications.

3D laser imaging technology could allow visualizing objects hidden in turbid water. Such a technology mainly works at short distances (<50 m) because of the high attenuation of light in water. Therefore, a significant part of the scattering events from the water column is located out of the optical depth of field (DoF), which could induce optical blur on images.

Discordance of PIK3CA mutational status between primary and metastatic breast cancer: a systematic review and meta-analysis.

Introduction: In light of the clinically meaningful results of the PI3K inhibitors in PIK3CA-mutated metastatic breast cancer (BC) patients, the reliable identification of PIK3CA mutations is of outmost importance. However, lack of evidence on the optimal site and timing of assessment, presence of temporal heterogeneity and analytical factors pose several challenges in clinical routine. We aimed to study the discordance rates of PIK3CA mutational status between primary and matched metastatic tumors.

Embryonic inhibition of colony-stimulating factor 1 receptor impacts craniofacial morphogenesis.

Objectives: Colony-stimulating factor-1 receptor (CSF1R) is vital for the recruitment of monocytes, and their proliferation and differentiation into functional osteoclasts. Mouse studies, where CSF1R and its cognate ligand are absent, have significant craniofacial phenotypes, but these have not been studied in detail.

Materials And Methods: Pregnant CD1 mice were fed diets laced with CSF1R inhibitor-PLX5622 starting at embryonic day 3.