Danger Biomarkers in Perfusates From Fatty Liver Grafts Subjected to Cold Storage Preservation in Different Preservation Solutions.

Static cold storage remains the traditional standard for liver graft preservation prior to transplantation in both clinical and experimental settings. The use of polyethylene glycol 35 solutions, such as Institut Georges Lopez-2 (IGL2) preservation solution, for protecting against mitochondrial damage during cold static preservation necessitates combination with hypothermic oxygenated perfusion to enhance liver graft performance. This study presents a preliminary comparative evaluation of "danger signals" indicating hepatocellular injury (transaminases, lactate content), mitochondrial damage (glutamate dehydrogenase release), and cytokine release in liver perfusates from suboptimal grafts (fatty livers) subjected to 24-hour cold storage.

Eicosanoid production, metabolism and contractile activity in the isolated uterus from non-insulin-dependent diabetic rats during late pregnancy.

Eicosanoid production, glucose (Glu), glycogen (Gly) and triglyceride (TG) metabolism, spontaneous contractile activity, PGF2 alpha and oxytocin-induced contractions have been studied in uterine tissue obtained from control (C) and non-insulin-dependent diabetic (D) rats prior to parturition. Parturition occurs on day 22 of gestation in control animals, whereas a 24 hr delay was observed in diabetic rats. Production of PGE2, PGE1, 6-keto-PGF1 alpha, PGF2 alpha, TXB2 and LTB4 was similar in uterine tissue obtained from control and diabetic rats on day 21 of pregnancy.

Eicosanoid production and phospholipase A2 activity in uterine tissue from castrated rats with non-insulin dependent diabetes mellitus.

In uterine tissue obtained from castrated control and non-insulin dependent diabetic (NIDDM) rats, eicosanoid production and its regulation by glucose levels and by the activity of phospholipase A2 (PLA2) was assessed. Basal outputs of prostaglandins (PGs) PGE2, PGE1, PGF2 alpha, 6-keto-PGF1 alpha (indicating the production of prostacyclin), thromboxane B2 (TXB2) (indicating the generation of TXA2) and leukotriene B4 (LTB4) were similar in control and NIDDM uterine preparations as assessed by RIA. When uterine conversion of labelled arachidonate into different prostanoids was evaluated, generation of 6-keto-PGF1 alpha, PGE2 and PGF2 alpha was similar in control and NIDDM uterine tissue, while TXB2 production was higher in the diabetic group.

Glucose, glycogen and triglyceride metabolism, as well as prostaglandin production in uterine strips and in embryos from diabetic pregnant rats. Influences of the presence of substrate in the incubation medium.

"In vitro" isometric developed tension (IDT) and frequency of contractions (FC), glucose (Glu), glycogen (GLY) and triglyceride (TG) metabolism, as well as prostaglandin PGE2 and PGE1 production, were studied in uterine strips and in embryos isolated from controls and diabetic rats at day 10 of pregnancy. The IDT and the FC, at 0 time or after a 60 min incubation, were not different in controls and in preparations from diabetic animals when the uterine strips were incubated in glucose or in glucose-free medium (p > 0.05).

Eicosanoid production by uterine strips and by embryos obtained from diabetic pregnant rats.

Eicosanoid production by uterine strips and by embryos obtained from normal and diabetic rats at day 10 of pregnancy was studied. It was found that the release of 6-keto-PGF1 alpha (representing PGI2 synthesis) and of LTB4 was less in preparations from diabetic animals than in controls. The production of TXB2 (indicating the formation of TXA2) by uterine tissue obtained from diabetic rats was almost double that of controls.