A paired study comparing mononuclear cell collection using a new in-line system with an off-line extracorporeal photopheresis apheresis system.

Background: Extracorporeal photopheresis (ECP) product characteristics are not well established. The aim of this study was to compare mononuclear cells (MNCs) collection using the new Amicus blue (AB) In-line ECP system to our standard Off-line ECP system using the Optia apheresis device and the MacoGenic G2 inactivation system (OM).

Study Design And Methods: We assessed the ECP products and procedure parameters, patient characteristics, and adverse events for both AB and OM systems in paired patients.

: gradient-free, spontaneous and controllable droplet motion on soft solids.

Most passive droplet transport strategies rely on spatial variations of material properties to drive droplet motion, leading to gradient-based mechanisms with intrinsic length scales that limit the droplet velocity or the transport distance. Here, we propose droplet , a novel mechanism that leverages an anisotropic fiber-reinforced deformable solid to achieve spontaneous and gradient-free droplet transport. Using high-fidelity simulations, we identify the fluid wettability, fiber orientation, anisotropy strength and elastocapillary number as critical parameters that enable controllable droplet velocity and long-range droplet transport.

Nicodemo's method on dental development: a cross-sectional study with 3,271 children and adolescents.

Civil and criminal forensics utilize dental development to estimate age. The method of Nicodemo, Moraes, and Médici Filho (NMM) is a popular dental age estimation tool in South America; however, it lacks a scientific basis for applications in contemporary forensic practice. This research included the largest sample ever collected in Brazil for a similar purpose.

Quantitative structural organization of the sclera in chicks after deprivation myopia measured with second harmonic generation microscopy.

Visual deprivation causes enhanced eye growth and the development of myopia, which is associated with a change in the arrangement of collagen fibers within the sclera. A second harmonic generation (SHG) microscope has been used to image the collagen fibers of unstained scleral punches from the posterior part of chicken eyes. We aimed to analyze the fibrous scleral tissue and quantify the changes in collagen organization in relation to the extent of induced deprivation myopia.

AMIC achieves sustained clinical improvement in isolated patellar cartilage defects over 5 years, correlating with MRI.

Purpose: To evaluate 5-year postoperative clinical outcomes of autologous matrix-induced chondrogenesis (AMIC) for isolated ICRS grade 3-4 patellar cartilage defects and correlate outcomes with magnetic resonance imaging (MRI). The hypothesis was that AMIC would improve clinical symptoms and induce neocartilage formation, visible on MRI, making it a safe and effective option for repairing focal patellar cartilage defects.

Methods: The cohort comprised 13 focal patellar lesions in 12 patients.