Accelerating CAR T cell manufacturing with an automated next-day process.

The traditional method of CAR T cell production involves lengthy ex-vivo culture times which can result in the reduction of crucial naïve T cell subsets. Moreover, traditional CAR T cell therapy manufacturing processes can prolong time-to-patient, potentially delaying patient treatment, and contribute to disease progression. In this study, we describe an innovative and semi-automated 24-hour CAR T manufacturing process that yields a higher percentage of naïve/stem-cell like T cells which showed high cytotoxic activity and cytokine release in vitro.

Landscape of antisense genes in the human genome and identification of new human hepatic antisense RNAs by long-read sequencing.

Background: Protein-coding genes have been considered the functional part of the genome, although they represent only 2% of the genome. In contrast, more than 90% of the genome produces non-coding RNA (ncRNA), including antisense (AS) genes, a type of long non-coding genes (encoding transcripts > 200 nucleotides) located on the opposite strand of coding genes. Therefore, antisense RNA (asRNA) can be complementary to the counterpart sense RNA, supporting a regulatory role with potential pathogenic consequences, as their deregulation has been associated with cardiovascular disease, cancer, and diabetes.

Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions.

Background: Antimicrobial-resistant bacteria represent a serious threat to public health. Among these bacteria, is of high priority because of its morbidity levels and its ability to induce different types of cancer.

Aim: This study aimed to identify strains encoding genes linked to the promotion of precancerous lesions and to isolate a bacteriophage to evaluate its preclinical potential against these bacteria.

Effect of insulin on indices of cerebral blood flow and cerebrovascular compliance in young adults.

Insulin has important vasodilatory effects in the peripheral circulation, but less is known about insulin's role in cerebrovascular control. Herein, we hypothesized both systemic (intravenous) and local (intranasal) insulin administration would increase indices of cerebral blood flow and reduce cerebrovascular compliance (Ci) in young adults. Participants were assigned to one of four separate protocols.