[Intrathyroidal iodine concentration after application of non-ionic contrast media with and without prophylactic application of perchlorate].

Aim: This study was designed to show the effect of a nonionic contrast medium (Iomeprol-300; CM) on the intrathyroidal iodine concentration with and without a concomitant medication with perchlorate (1380 mg/d) to block the thyroidal iodine uptake.

Volunteers And Methods: Twelve volunteers recieved 100 ml Iomeprol-300 intravenously and the perchlorate prophylaxis mentioned above. Another 12 volunteers got 100 ml Iomeprol-300 only.

Real time RT-PCR analysis of thyroglobulin mRNA in peripheral blood in patients with congenital athyreosis and with differentiated thyroid carcinoma after stimulation with recombinant human thyrotropin.

Objective: Thyroglobulin (Tg), measured by immunometric assay, is the most sensitive and widely used clinical marker for thyroid cancer progression and relapse. However, these Tg determinations are of limited sensitivity and susceptible to interference by Tg autoantibodies. As a possible diagnostic alternative, we tested a real time RT-PCR protocol to determine Tg mRNA levels in peripheral blood.

[Guideline for in vivo- and in vitro procedures for thyroid diseases (version 2)].

The version 2 of the guideline for diagnostic standards of thyroid disorders is an update of the guideline published in 1999 and describes standards of in vitro and in vivo procedures. The following statements are modified: In vitro procedures: When measurement of the TSH-receptor antibodies is indicated, the guideline recommends the use of a second generation assay (recombinant human TSH-receptor as antigen). The functional assay sensitivity for the measurement of thyroglobulin should reach a value < or =1 ng/ml.

Technical evaluation of a new immunoradiometric and a new immunoluminometric assay for thyroglobulin.

Background: After removal of differentiated thyroid carcinoma (DTC), serum thyroglobulin (Tg) can indicate persistent or recurrent disease. We describe two novel two-step assays designed to measure low Tg concentrations.

Methods: We evaluated prototypes of the new IRMA, DYNOtest Tg-pluS, and the new immunoluminometric assay (ILMA), LUMItest) Tg-pluS.

L-arginine deficiency and supplementation in experimental acute renal failure and in human kidney transplantation.

Background: The "L-arginine paradox" refers to situations where L-arginine (L-Arg) supplementation stimulates nitric oxide (NO) synthesis, despite saturating intracellular concentrations. This paradox is frequently observed in acute renal failure (ARF). First, the effects of L-Arg on renal function of rats with ARF were studied.