High Dynamic Range Digital Assay Enabled by Dual-Volume Centrifugal Step Emulsification.

We implement dual-volume centrifugal step emulsification on a single chip to extend the dynamic range of digital assays. Compared to published single-volume approaches, the range between the lower detection limit (LDL) and the upper limit of quantification (ULQ) increases by two orders of magnitude. In comparison to existing multivolume approaches, the dual-volume centrifugal step emulsification requires neither complex manufacturing nor specialized equipment.

Physical activity and the frequency of acute exacerbations in patients with chronic obstructive pulmonary disease.

Purpose: Acute exacerbations (AE) in patients with COPD are associated with a decline in lung function, increased risk of hospitalization, and mortality. In this cross-sectional study we tested whether the level of objectively measured daily physical activity and exercise capacity are associated with the number of COPD exacerbations.

Methods: In 210 patients with COPD (67 % men; mean (SD) age: 63 (8) years) enrolled in The Obstructive Pulmonary Disease Outcomes Cohort of Switzerland (TOPDOCS) physical activity (PA) (steps per day, physical activity level, (PAL)), exercise capacity (6-min walking distance, (6MWD)), comorbidities, lung function, and medication were assessed.

Impact of Nurse-Regulated Feedings on Growth Velocity and Weight Gain of 1200-1500 g Preterm Infants.

Purpose: To evaluate the impact of nurse-regulated feedings (NRFs) on growth velocity and weight gain of 1200-1500 g preterm infants.

Subjects: Cohort 1: All preterm infants 1200-1500 g between 1997 and 2001 not on NRF protocol; Cohort 2: All preterm infants 1200-1500 g between 2003 and 2006 on NRF protocol. Both cohorts screened out for small gestation age, major congenital anomalies, intraventricular hemorrhage (IVH), necrotizing enterocolitis (NEC), culture positive infection at birth, meningitis, and those requiring surgery.

Dynamics in the ultrastructure of asymmetric axospinous synapses in the frontal cortex of hibernating European ground squirrels (Spermophilus citellus).

Recent theories on the function of arousals from torpor in hibernating mammals focus on the repair of the central nervous system from damage accumulating during prolonged hypothermia. In this framework, we investigated the synaptic ultrastructure in Layer 2 of the frontal cortex from hibernating European ground squirrels (Spermophilus citellus) sacrificed at four different phases in the torpor-arousal cycle. Using electron microscopy, we quantified synapse number and morphometric data on asymmetric axospinous synapses.