Publications by authors named "J R Wireman"
Article Synopsis
- Recent advancements in rapid high-throughput sequencing have raised hopes for whole genome sequencing (WGS) to fully characterize antibiotic resistance (AMR) in bacteria, but challenges remain for clinical application.
- The study compared methods for detecting AMR genes in three ESKAPE bacteria, suggesting that total genomic DNA can suffice for detection without the need for separate plasmid DNA purification.
- Findings indicate that using a combination of Illumina short-reads and ONT long-reads improves the sensitivity and accuracy of AMR gene detection, but the effectiveness varies among different AMR databases.
Article Synopsis
- Multi-antibiotic resistant (MAR) bacteria result in significant healthcare costs and loss of life, raising concerns about antibiotic misuse and the need for new antibiotic discovery.
- The role of mobile genetic elements (MGEs) in generating MAR through the acquisition of resistance genes is highlighted, emphasizing the need to investigate and potentially disable these elements.
- Exposure to non-antibiotic toxic metals, especially mercury, has been shown to co-select for MAR bacteria, suggesting that reducing exposure to such metals could lead to decreased MAR prevalence in public health.
Plasmids are important in evolution and adaptation of host bacteria, yet we lack a comprehensive picture of their own natural variation. We used replicon typing and RFLP analysis to assess diversity and distribution of plasmids in the ECOR, SARA, SARB and SARC reference collections of Escherichia coli and Salmonella. Plasmids, especially large (≥30 kb) plasmids, are abundant in these collections.
View Article and Find Full Text PDFThrough the National Center for Integrated Civilian-Military Domestic Disaster Medical Response, the Yale New Haven Center for Emergency Preparedness and Disaster Response and US Northern Command have initiated a study to determine the requirements of a national operational epidemiological modelling process. During a public health emergency, decision makers often require a variety of epidemiological information, including prospective forecasts that can be made available in the form of models. Currently, there is no formal process across US Government agencies and departments to synchronise epidemiological models with response organisations.
View Article and Find Full Text PDFArticle Synopsis
- * A significant 79% of these strains had large plasmids (over 20 kb), with most of these ranging from 20-30 kb in size, and they often carried multiple resistance and virulence genes.
- * Researchers sequenced 93 complete and 57 partial plasmids, significantly increasing the available data, and found that plasmids from different time periods and locations were over 98% identical, indicating strong selective pressure to maintain certain genetic traits.