Insights into conformational ensembles of compositionally identical disordered peptidomimetics.

While the conformational ensembles of disordered peptides and peptidomimetics are complex and challenging to characterize, they are a critical component in the paradigm connecting macromolecule sequence, structure, and function. In molecules that do not adopt a single predominant conformation, the conformational ensemble contains rich structural information that, if accessible, can provide a fundamental understanding related to desirable functions such as cell penetration of a therapeutic or the generation of tunable enzyme-mimetic architecture. To address the fundamental challenge of describing broad conformational ensembles, we developed a model system of peptidomimetics comprised of polar glycine and hydrophobic -butylglycine to characterize using a suite of analytical techniques.

The Salford Nature Environments Database (SNED): an open-access database of standardized high-quality pictures from natural environments.

The growing interest in harnessing natural environments to enhance mental health, including cognitive functioning and mood, has yielded encouraging results in initial studies. Given that images of nature have demonstrated similar benefits, they are frequently employed as proxies for real-world environments. To ensure precision and control, researchers often manipulate images of natural environments.

Demystifying environmental health-related diseases: Using ICD codes to facilitate environmental health clinical referrals.

The burden of disease of Aboriginal and Torres Strait Islander people is estimated as 2.3 times that of the broader Australian population, with between 30% and 50% of health inequalities attributable to poor environmental health. Although many Australian states and territories have clinical policy initiatives that seek to reduce the burden of preventable disease in this population, including field-based environmental health clinical referrals (EHCRs), there is little consistency across the jurisdictions, resulting in less potential to break the cycle of recurrent diseases within the home environment.

A High-Throughput Workflow to Analyze Sequence-Conformation Relationships and Explore Hydrophobic Patterning in Disordered Peptoids.

Understanding how a macromolecule's primary sequence governs its conformational landscape is crucial for elucidating its function, yet these design principles are still emerging for macromolecules with intrinsic disorder. Herein, we introduce a high-throughput workflow that implements a practical colorimetric conformational assay, introduces a semi-automated sequencing protocol using MALDI-MS/MS, and develops a generalizable sequence-structure algorithm. Using a model system of 20mer peptidomimetics containing polar glycine and hydrophobic -butylglycine residues, we identified nine classifications of conformational disorder and isolated 122 unique sequences across varied compositions and conformations.