Publications by authors named "J R Calarco"
Tissue-regulated alternative exons are dictated by the interplay between cis-elements and trans-regulatory factors such as RNA binding proteins. Despite extensive research on splicing regulation, the full repertoire of these cis and trans features and their evolutionary dynamics across species are yet to be fully characterized. Members of the CUG-binding protein and ETR-like family (CELF) of RNA binding proteins are known to play a key role in the regulation of tissue-biased splicing patterns, and when mutated, these proteins have been implicated in a number of neurological and muscular disorders.
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- Reliable methods for detecting gene expression are crucial for studying development and responses to genetic/environmental changes.
- Caenorhabditis elegans is a valuable model organism due to its sequenced genome, transparent body, and available genetic tools, making it easier to connect gene expression to cellular characteristics.
- The text reviews various techniques for analyzing gene expression in C. elegans, including bulk RNA sequencing, single-cell RNA sequencing, and proteomics, as well as resources for accessing gene expression data.
Article Synopsis
- A new robotic system has been developed to enable accurate 3D imaging and morphological profiling of small model organisms like nematodes, overcoming challenges in resolution and complex shapes.
- The system allows for contactless rotation and high-resolution imaging, which provides detailed 3D reconstructions at various developmental stages and mutant strains.
- This technological advancement has led to the discovery of genetic interactions relevant to neurological and muscular disorders, showcasing its potential for broader applications in studying various biological samples.
The nematode Caenorhabditis elegans is widely employed as a model organism to study basic biological mechanisms. However, transgenic C. elegans are generated by manual injection, which remains low-throughput and labor-intensive, limiting the scope of approaches benefitting from large-scale transgenesis.
View Article and Find Full Text PDFComparison of methods proposed for monitoring cefotaxime-resistant in the water environment.
Appl Environ Microbiol
May 2024
is a promising subject for globally coordinated surveillance of antimicrobial resistance (AMR) in water environments due to its clinical relevance and widespread use as an indicator of fecal contamination. Cefotaxime-resistant was recently evaluated favorably for this purpose by the World Health Organization TriCycle Protocol, which specifies tryptone bile x-glucuronide (TBX) medium and incubation at 35°C. We assessed comparability with the U.
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