Whole genome sequencing approaches for taxonomic profiling and evaluation of wastewater quality.

Tracking metagenomic abundance in wastewater is undoubtedly a powerful tool to detect emerging variants and improve community health. However, there are a few factors that limit environmental water-based genomic monitoring: sampling variability, incomplete coverage, genetic fragmentation, degradation, data analysis and interpretation. The decreasing costs of high-throughput sequencing and high-end supercomputers have increased the use and accuracy of genomic data for microbial detection and monitoring in wastewater samples within any given region.

Detection and subtyping of influenza A virus in porcine clinical samples from Spain in 2020.

A total of 1019 samples collected on 726 Spanish swine farms suffering from outbreaks of respiratory disease were screened for influenza A viruses (IAVs) using a RT-qPCR method. A subset of positive samples was further analyzed using a subtype-specific RT-qPCR method (n: 142) and Sanger sequencing (n: 64). A total of 19.

Subtype Distribution of spp. in Patients with Gastrointestinal Symptoms in Northern Spain.

Limited molecular data exist on the prevalence and subtype distribution of spp., the most prevalent parasite in human and animal feces worldwide. A total of 44 different subtypes (STs) of are currently recognized based on the sequence of the small subunit ribosomal RNA () gene.

Molecular Diagnosis of Footrot and Contagious Ovine Digital Dermatitis in Small Ruminants in the Iberian Peninsula.

Contagious ovine digital dermatitis (CODD) and footrot (FR), a sub-acute or acute necrotic (decaying) infectious disease involving the hoof and underlying tissues, pose economic challenges to herds in Spain and worldwide. The aetiological agent for FR is , while CODD is caused by pathogenic phylogroups. We detail the findings derived from the analysis by qPCR of 105 pooled samples from 100 ovine and five caprine herds in Spain and Portugal, alongside 15 samples from healthy flocks in order to identify , , spp.

Reverse impression technique: A fully digital protocol for the fabrication of the definitive fixed prosthesis for completely edentulous patients.

In complete arch implant rehabilitation, one of the greatest difficulties still encountered in the digital workflow is the deviation of the implant position during intraoral scanner (IOS) data acquisition. As a result, the passivity of a definitive prosthesis fabricated using IOS data might be compromised. Thus, an implant position verification method is essential, either digitally or conventionally executed.