Generation of novel bpm6 and dmr6 mutants with broad-spectrum resistance using a modified CRISPR/Cas9 system in Brassica oleracea.

Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene BoBPM6 and the DOWNY MILDEW RESISTANCE 6 gene in Brassica oleracea resulted in new lines with broad-spectrum disease resistance.

Erratum to "Sleep Promotion by 3-Hydroxy-4-iminobutyric Acid in Walnut ".

[This corrects the article DOI: 10.34133/research.0216.

MELK prevents radiofrequency ablation-induced immunogenic cell death and antitumor immune response by stabilizing FABP5 in hepatocellular malignancies.

Background: Radiofrequency ablation (RFA) is an efficient treatment with unlimited potential for liver cancer that can effectively reduce patient mortality. Understanding the biological process related with RFA treatment is important for improving treatment strategy. This study aimed to identify the critical targets for regulating the efficacy of RFA.

Accuracy of the over-preparation technique in implant surgery in the maxillary anterior region: an in vitro study.

Background: In the maxillary anterior region, differences in bone density along the drilling path can impact the accuracy of implant placement despite the use of a surgical guide. Hence, the aim of this in vitro study was to investigate the accuracy of implant placement using the over-preparation technique in different drilling environments in the maxillary anterior region.

Methods: Three experimental models (a, b, and c) were designed and fabricated to simulate the following drilling environments (n = 60 each): unhealed bone, less dense bone, and dense bone after tooth extraction.

Biochemical, structural, and cellular characterization of S-but-3-yn-2-ylglycine as a mechanism-based covalent inactivator of the flavoenzyme proline dehydrogenase.

The mitochondrial flavoenzymes proline dehydrogenase (PRODH) and hydroxyproline dehydrogenase (PRODH2) catalyze the first steps of proline and hydroxyproline catabolism, respectively. The enzymes are targets for chemical probe development because of their roles in cancer cell metabolism (PRODH) and primary hyperoxaluria (PRODH2). Mechanism-based inactivators of PRODH target the FAD by covalently modifying the N5 atom, with N-propargylglycine (NPPG) being the current best-in-class of this type of probe.