Publications by authors named "J Pigac"
Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3,4-dichloroisocoumarin.
Acta Crystallogr Sect F Struct Biol Cryst Commun
November 2011
A recombinant lipase (triacylglycerol acylhydrolase; EC 3.1.1.
View Article and Find Full Text PDFGenetics of Streptomyces rimosus, the oxytetracycline producer.
Microbiol Mol Biol Rev
September 2006
Article Synopsis
- Streptomyces rimosus is a well-studied bacterium known for producing oxytetracycline and other tetracycline antibiotics, which are important despite decreasing clinical use due to resistance.
- Genetic manipulation techniques have been developed since the 1950s to investigate genetic instability in S. rimosus and to identify genes related to oxytetracycline production.
- Recent improvements in genome sequencing technology may allow researchers to obtain the complete genome sequence of S. rimosus soon.
The cloning, sequencing and high-level expression of the gene encoding extracellular lipase from Streptomyces rimosus R6-554W have been recently described, and the primary structure of this gene product was deduced using a bioinformatic approach. In this study, capillary electrophoresis-on-the-chip and mass spectrometry were used to characterize native and overexpressed extracellular lipase protein from S. rimosus .
View Article and Find Full Text PDFArticle Synopsis
- A new extracellular lipase from Streptomyces rimosus R6-554W has been isolated and characterized, with its gene cloned and sequenced, revealing an open reading frame (ORF) of 804 bp that translates to a polypeptide of 268 amino acids.
- The mature lipase has a theoretical molecular mass of 24.172 kDa, confirmed experimentally, but displays no significant similarity to other known lipases, showing some relatedness to hypothetical proteins in Streptomyces coelicolor A3(2) and esterases in other species.
- This lipase is part of family II of lipolytic enzymes, which was previously unrecognized in the Streptomyces genus, and
A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation.
Appl Environ Microbiol
January 1995
Usually plasmid DNA is introduced into Streptomyces strains by polyethylene glycol-mediated transformation of protoplasts. However, many Streptomyces strains are only poorly or not at all transformable via protoplasts. Therefore, we have optimized the parameters critical for the application of electrotransformation of plasmid DNA into Streptomyces species.
View Article and Find Full Text PDF