Correlation of extracts obtained by high efficiency gel chromatography of lactic dehydrogenase virus infected mouse serum and cytosol from human tumors using leukocyte adherence inhibition assay.

Immunochemically active fractions were obtained using Separon Hema-300-glc(R) from serum of lactic dehydrogenase virus (LDV) infected mice and from homogenates of human tumors. The mixture of proteins of tumorous origin from the cytosol giving a positive reaction in the leukocyte adherence inhibition (LAI) test was found in the same fractions showing maximum of absorbance at 340 nm in the spectrophotometer and a corresponding peak in the refractometer. Analogous peaks were not proved in material obtained from healthy controls, but they were found in some human placentas and fetal organs.

Isolation of immunoreactive components from experimental and human tumour tissues and serums by high-performance gel chromatography.

A method for the rapid separation of proteinous fractions by high-performance gel chromatography was described. Homogenates from tumorous and healthy tissues and blood were eluted by saline on a column packed with rigid hydrophilic macroporous particles of O-glucose--Spheron 300. Fractions were collected and subjected to further analyses.