Anti-pokeweed mitogen antiserum inhibits and enhances blastogenesis of mononuclear cells induced by pokeweed mitogen.

The interaction between pokeweed mitogen (PWM) and peripheral blood mononuclear cells (PBMC) was investigated using rabbit anti-PWM antiserum (anti-PWM) and 125I-PWM. Incubation of PBMC with PWM in the presence of anti-PWM resulted in an inhibition of the mitogenic effect of PWM. Anti-PWM predominantly blocked the interaction of PWM with monocytes, which is essential for optimal stimulation of lymphoid cells with PWM.

Measurement of single-cell DNA synthesis by pokeweed mitogen-stimulated mononuclear cells with combined light and scanning electron microscopy.

The response of peripheral blood mononuclear cells to pokeweed mitogen (PWM) stimulation was investigated with combined light (LM) and scanning electron microscopy (SEM). The DNA content of the nucleus of a cell (measured by fluorescence) was compared directly with the diameter and morphological features (by SEM). It was found that upon PWM stimulation lymphocytes transform into blast cells without measurable increase of diploid DNA content (4C) were not seen.

Electronmicroscopical observations on monocyte-lymphocyte interactions upon stimulation with pokeweed mitogen latex conjugate.

In this study we report on the preparation and application of pokeweed mitogen (PWM) conjugated to latex particles. This conjugate (PWM-latex) was prepared by incubation of PWM with latex particles in the presence of glutaraldehyde. The effect of the addition of PWM-latex to human peripheral blood mononuclear cells (PBMC) was fully comparable to the addition of PWM alone i.

The use of a peroxidase-anti-peroxidase complex for the visualization of monoclonal antibodies on the ultrastructural level.

Human peripheral blood mononuclear cell (PBMC) were treated with a panel of monoclonal antibodies (MoAbs) for the demonstration of membrane antigens at the ultrastructural level. The bound MoAbs were linked by rabbit anti-mouse IgG to a peroxidase-anti-peroxidase (PAP) complex composed of monoclonal mouse anti-peroxidase antibodies and horse radish peroxidase. This labelling method with a three step incubation procedure resulted in clear demonstration of the membrane antigens.

Morphometric analysis of peripheral blood and synovial fluid lymphocytes of patients with rheumatic disease.

A morphometric analysis of lymphocytes from the peripheral blood and the synovial fluid (SF) of patients with rheumatoid arthritis (RA) was based on a determination of the nuclear contour index (NCI = perimeter divided by the square root of the area of the nucleus). Use of this method showed that a particular type of lymphocyte, the cerebriform mononuclear cell (CMC), occurred in higher percentages in the SF than in the peripheral blood of patients with RA. The mean NCI of the lymphocytes (non-CMC) was also higher in the SF.