Influence of a 12-hour, 22 degrees C holding period for buffy coats on the preparation of platelet concentrates stored in plasma.

Background: The preparation of platelet concentrates (PCs) from buffy coats (BCs) stored at room temperature is controversial, because of the strong metabolic activity of cells in BCs and the possible detrimental effect of neutrophil enzymes on platelets when the holding time before separation is prolonged. Despite good in vitro and in vivo behavior of BC-PCs stored in synthetic solution, little is known of the quality of BC-PCs stored in plasma.

Study Design And Methods: Comparison was made of PCs prepared from BCs held at 22 degrees C for 3 hours (3-hour BC-PCs) or overnight (12-hour BC-PCs) and stored in plasma.

[Anti-HBc in blood donors. Distribution by sex and age classes].

Among the 21,341 blood donors who gave their blood in 1990, 638 were anti-HBc positive. There is a significant difference between men and women who are 18 to 22 years old and those who are more than 50 years old. In the first case, this difference can be referred to the heterogeneity of the male population and in the second case, to the aftermath of military campaigns.

[Flow cytometric analysis of cellular DNA content in Barret's esophagus. A study of 66 cases].

Dysplasia is the only marker for malignant potential in Barrett's esophagus. The histologic interpretation of dysplasia is sometimes difficult, particularly when attempting to distinguish dysplastic changes from those of a regenerating and inflammatory mucosa. In order to find an objective marker to identify patients with high risk of malignant transformation, the authors evaluated 497 biopsies from 66 patients with Barrett's esophagus with flow cytometry.