Effect of mechanical traumatization on rate and constancy of oxygen uptake of isolated diaphragms.

Diaphragms of mice were traumatized by division parallel or transversally to the muscle fibres. The extent of traumatization was varied by the degree of subdivision. Oxygen uptake was measured by the Warburg technique.

Is oxygen consumption of surviving tissues determined by the oxygen tension of the suspension medium?

Oxygen tension in the suspension medium and oxygen uptake of the isolated diaphragm of the mouse have been determined simultaneously with systematic variation of shaking frequency and oxygen concentration of the gas phase. Oxygen tension in the suspension medium reaches its final value during the usual equilibration period of 10 minutes and remains constant for the whole measuring period. Each combination of shaking frequency and oxygen concentration of the gas phase results in a reproducible value of oxygen tension in the medium.

[The influence of mode and intensity of homogenization on the absolute value and stability of oxygen consumption of guinea pig liver homogenates (author's transl)].

The influence of five different methods of homogenisation (1. The method according to Potter and Elvehjem, 2. A modification of this method called Potter S, 3.