AFNOR validation of Premi Test, a microbiological-based screening tube-test for the detection of antimicrobial residues in animal muscle tissue.

Premi Test contains viable spores of a strain of Bacillus stearothermophilus which is sensitive to antimicrobial residues, such as beta-lactams, tetracyclines, macrolides and sulphonamides. The growth of the strain is inhibited by the presence of antimicrobial residues in muscle tissue samples. Premi Test was validated according to AFNOR rules (French Association for Normalisation).

Elimination of aminoglycoside antibiotics in milk following intramammary administration.

The elimination in cow milk of aminoglycoside antibiotic residues (neomycin, dihydrostreptomycin, kanamycin and gentamicin) was studied after intramammary administration of eight drug formulations marketed in France. Quantitative residue analysis was performed by a cylinder plate method. The sensitivity was 0.

Residues of macrolide antibiotics in eggs following medication of laying hens.

1. The elimination kinetics of four macrolide antibiotics (tylosin, erythromycin, spiramycin and josamycin) in eggs were determined separately for albumen and yolk after oral administration through either drinking water or diet or after intramuscular injection. 2.

Sulphonamide residues in milk of dairy cows following intravenous injection.

An investigation was conducted into the elimination into milk of four sulphonamides (sulphadimidine, sulphamethoxypyridazine, sulphadimethoxine and sulphadoxine) after intravenous injections of drugs available in France. Sulphonamide determination in milk was performed using a high-performance liquid chromatography (HPLC) method with detection limit of 0.01 micrograms/ml.

Excretion of penicillins and cephalexin in bovine milk following intramammary administration.

The elimination into bovine milk of beta-lactam antibiotic residues (procaine penicillin G, cloxacillin, ampicillin, oxacillin, cephalexin) following intramammary administration of 10 preparations marketed in France was studied. The quantitative analysis of residues was carried out by a microbiological agar diffusion method using Bacillus stearothermophilus. Sensitivity ranged from 0.