Combined rotavirus and K99 Escherichia coli infection in gnotobiotic pigs.

Fifty nine 3-day-old gnotobiotic pigs were randomly assigned to 4 experimental groups: 14 pigs were orally inoculated with rotavirus (RV), 14 were orally inoculated with enterotoxigenic Escherichia coli (ETEC), 18 were orally inoculated with both agents, and 13 were controls. Pigs inoculated with RV plus ETEC were given the RV inoculum at 3 days of age and then, 24 hours later, were given the ETEC inoculum. Three pigs inoculated only with RV, 3 pigs inoculated only with ETEC, 4 pigs inoculated with RV plus ETEC, and 3 pigs in the control group were euthanatized at 5 and 7 days of age.

Bovine herpesvirus-5 (DN599) antigens in cells derived from bovine ocular squamous cell carcinoma.

Bovine herpesvirus 5 antigens were detected by indirect immunofluorescence in the cytoplasm of tumor cells derived from bovine ocular squamous cell carcinomas. The number of tumor cells expressing bovine herpesvirus 5 antigens was increased following exposure to ultraviolet light or maintenance in pH 8.4 media.

Shedding of rotavirus in feces of sows before and after farrowing.

The purpose of this study was to determine whether sows shed rotavirus near the time of farrowing. Twelve sows purchased from a common source and seropositive for rotavirus were housed in isolation in farrowing crates from 5 days before to 3 weeks after farrowing. Fecal samples were collected at 3- to 4-day intervals and examined for the presence of rotavirus by direct electron microscopy.

Experimental infection of conventional dogs with canine parvovirus.

Four 6-week-old conventional pups were inoculated with a parvovirus (PV) isolated from the feces of a dog with naturally occurring enteritis. Blood for hematologic studies, virus isolation (VI), and antibody titration and feces for VI and negative-contrast electron microscopy were collected on day 0 and daily until necropsy. Beginning at postinoculation day 2, necropsies were done and specimens were collected for immunofluorescence, VI, and light microscopic examination.

Recrudescence of bovine herpesvirus-5 in experimentally infected calves.

A latent infection of bovine herpesvirus-5 (BHV-5) was established in 4 calves. These calves, plus 2 controls, were given dexamethasone (DM) to reactivate the latent virus. The 4 principal calves developed antibodies to BHV-5 by postinoculation day (PID) 21.