Dietary supplementation with 15% tomato pomace (Solanum lycopersicum L.) improves sperm production and antioxidant status in aged male broiler breeders.

The objective was to investigate effects of dietary tomato pomace (TP) and L-Arginine (L-Arg; 10% above nutritional recommendation) in aged male broiler breeders. Thirty male broiler breeders (Ross 308), 58 wk old, were randomly and equally allocated into five experimental groups: basal diet (C); 5% TP-supplemented (T-5); 10% TP-supplemented (T-10); 15% TP-supplemented (T-15); and L-Arg supplemented (L-10) groups. In contrast to the Control and TP-supplemented groups, sperm of L-10 roosters had higher C14:1, C16:0 and C18:1 n-9c proportions, total saturated fatty acid (FA) contents, and n-6:n-3 ratios, but a lower C22:4 n-6 content (P<0.

Transcriptomics of bovine sperm and oocytes.

Traditionally, sperm and embryos were studied using microscopy to assess morphology and motility. However, OMICS technologies, especially transcriptomic analysis, are now being used to screen the molecular dynamics of fertility markers at cellular and molecular levels, with high sensitivity. Transcriptomics is the study of the transcriptome - RNA transcripts produced by the genome - using high-throughput methods to understand how the RNAs are expressed.

Landscape transcriptomic analysis of bovine follicular cells during key phases of ovarian follicular development.

Background: There are many gaps in our understanding of the mechanisms involved in ovarian follicular development in cattle, particularly regarding follicular deviation, acquisition of ovulatory capacity, and preovulatory changes. Molecular evaluations of ovarian follicular cells during follicular development in cattle, especially serial transcriptomic analyses across key growth phases, have not been reported. This study aims to address this gap by analyzing gene expression using RNA-seq in granulosa and antral cells recovered from ovarian follicular fluid during critical phases of ovarian follicular development in Holstein cows.