Publications by authors named "J P Huggins"
Objective: To develop a Diagnostic and Statistical Manual of Mental Disorders (DSM-5) eating disorder screener.
Method: Veterans enrolled in VA healthcare (N = 344) completed a survey of screening items and established measures. A validation subset (n = 166) participated in diagnostic interviews to confirm an eating disorder diagnosis.
Objective: Syndrome of undifferentiated recurrent fevers (SURF) is characterized by recurrent fevers and autoinflammation without a confirmed molecular diagnosis of a hereditary recurrent fever syndrome, and not fulfilling criteria for periodic fever, adenitis, pharyngitis, aphthous stomatitis syndrome (PFAPA). The goal of this study was to characterize clinical features of patients with SURF compared to patients with PFAPA and to analyze their cytokine signature, genetic variations, and responses to treatment.
Methods: We enrolled 46 patients observed at Cincinnati Children's Hospital Medical Center.
Mutational processes, such as the molecular effects of carcinogenic agents or defective DNA repair mechanisms, are known to produce different mutation types with characteristic frequency profiles, referred to as mutational signatures. Non-negative matrix factorization (NMF) has successfully been used to discover many mutational signatures, yielding novel insights into cancer etiology and targeted therapies. However, the NMF model is only a rough approximation to reality, and even small departures from this assumed model can have large negative effects on the accuracy and reliability of the results.
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- - Genetically-encoded single-cell barcodes can help with tasks like tracing cell lineages and conducting genetic screens, but current methods have limitations in speed and effectiveness.
- - This study proposes a new method using combinations of fluorescent proteins to create a high-diversity barcode library that allows for non-destructive, quick, and affordable identification of cells.
- - The researchers successfully tested this method, creating a library of about 150 unique barcodes using 18 fluorescent proteins, and demonstrated its effectiveness for classifying cells and enabling genetic screenings and lineage tracing.
Objective: We compared the measurement properties of a traditional physician global assessment of disease activity (PhGA) 10-cm visual analog scale (PhGA) with that of the three-point numeric scale (PhGA) in childhood-onset systemic lupus erythematosus (cSLE) as part of the childhood Lupus Low Disease Activity State (cLLDAS).
Methods: We used a secondary data analysis from a convenience sample of 100 patients with cSLE followed every three months for up to seven visits. Ratings of PhGA, PhGA, parent assessment of patient well-being (ParGA) (range: 0= very poorly, 10 = very well), disease activity as measured by the SLE disease activity index 2000 (SLEDAI-2k), Safety of Estrogens in Lupus Erythematosus National Assessment (SELENA) SLEDAI, and the British Isles International Lupus Activity Group index (BILAG; A = 9, B = 3, C = 1, D/E = 0) were compared.