Understanding factors influencing community resilience to disturbance is critical for mitigating harm at various scales, including harm from medication to gut microbiota and harm from human activity to global biodiversity, yet there is a lack of data from large-scale controlled experiments. Factors expected to boost resilience include prior exposure to the same disturbance and dispersal from undisturbed patches. Here we set up an in vitro system to test the effect of disturbance pre-exposure and dispersal represented by community mixing.
View Article and Find Full Text PDFHybrid maize seed production in Africa is dependent upon manual detasseling of the female parental lines, often resulting in plant damage that can lead to reduced seed yields on those detasseled lines. Additionally, incomplete detasseling can result in hybrid purity issues that can lead to production fields being rejected. A unique nuclear genetic male sterility seed production technology, referred to as Ms44-SPT, was developed to avoid hybrid seed loss and to improve the purity and quality of hybrid maize production.
View Article and Find Full Text PDFSingle-cell MALDI mass spectrometry imaging (MSI) of lipids and metabolites >200 Da has recently come to the forefront of biomedical research and chemical biology. However, cell-targeting and metabolome-preserving methods for analysis of low mass, hydrophilic metabolites (<200 Da) in large cell populations are lacking. Here, the PRISM-MS (PRescan Imaging for Small Molecule - Mass Spectrometry) mass-guided MSI workflow is presented, which enables space-efficient single cell lipid and metabolite analysis.
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