Characterization of virus‒host recombinant variants of the hepatitis E virus.

Unlabelled: Hepatitis E virus is a single-strand, positive-sense RNA virus that can lead to chronic infection in immunocompromised patients. Virus-host recombinant variants (VHRVs) have been described in such patients. These variants integrate part of human genes into the polyproline-rich region that could introduce new post-translational modifications (PTMs), such as ubiquitination.

A fully automated system using transcription-mediated amplification for the molecular diagnosis of hepatitis E virus in human blood and faeces.

Background And Objectives: We evaluated the performance of the Procleix HEV RNA assay implemented on the Panther automated platform for detecting HEV RNA.

Study Design And Results: Analytical specificity was 100% and there was no cross contamination, as assessed by assaying 122 plasma samples from HEV RNA-negative blood donors. The limits of detection were determined by Probit analysis with the WHO HEV standard (HEV subtype 3a) and subtype 3f and 3c reference strains.

Detection and quantification of HIV-1 RNA with a fully automated transcription-mediated-amplification assay.

Background: Nucleic acid testing is the major method used to monitor HIV viral load. Commercial systems based on real-time PCR assays are available for high-volume centralized laboratory testing, but they are not fully automated.

Objectives And Study Design: We have compared the diagnostic performance of the Hologic Aptima HIV-1 Quant Dx assay (Aptima) (based on real-time TMA) on the Panther instrument, a fully-automated random access platform, to that of, the Roche Cobas Ampliprep Cobas TaqMan (CAP/CTM) HIV-1 version 2.

Analytical performance of the VERIS MDx system HCV assay for detecting and quantifying HCV RNA.

Background: The diagnosis of HCV relies on the detection of viral RNA.

Objective: To evaluate the performance of the VERIS/MDx System HCV Assay, a new automated system for quantifying HCV RNA, and to compare with the COBAS Ampliprep/COBAS Taqman™ (CAPCTM) HCV Test version 2.0.

Performance of a completely automated system for monitoring CMV DNA in plasma.

Background: Completely automated systems for monitoring CMV-DNA in plasma samples are now available.

Objectives: Evaluate analytical and clinical performances of the VERIS™/MDx System CMV Assay(®).

Study Design: Analytical performance was assessed using quantified quality controls.