Multilocus DNA sequencing of the whiskey fungus reveals a continental-scale speciation pattern.

was described to accommodate a single species, . Known as the 'whiskey fungus', this species is the predominant member of a ubiquitous microbial community known colloquially as 'warehouse staining' that develops on outdoor surfaces subject to periodic exposure to ethanolic vapours near distilleries and bakeries. Here we examine 19 strains recovered from environmental samples near industrial settings in North America, South America, the Caribbean, Europe and the Far East.

Ethanol physiology in the warehouse-staining fungus, Baudoinia compniacensis.

The fungus Baudoinia compniacensis colonizes the exterior surfaces of a range of materials, such as buildings, outdoor furnishings, fences, signs, and vegetation, in regions subject to periodic exposure to low levels of ethanol vapour, such as those in the vicinity of distillery aging warehouses and commercial bakeries. Here we investigated the basis of ethanol metabolism in Baudoinia and investigate the role of ethanol in cell germination and growth. Germination of mycelia of Baudoinia was enhanced by up to roughly 1d exposure to low ethanol concentrations, optimally 10ppm when delivered in vapour form and 5mm in liquid form.

The influence of sampling duration on recovery of culturable fungi using the Andersen N6 and RCS bioaerosol samplers.

Unlabelled: The influence of sampling duration on recovery of culturable fungi was compared using the Andersen N6 and the Reuter Centrifugal Sampler (RCS). Samplers were operated side-by-side, collecting 15 samples each of incrementally increasing duration (1-15 min). From 270 samples collected, 26 fungal genera were recovered.

Semiselective isolation of the ethanol-imbibing sooty mould Baudoinia of distillery aging warehouses.

Baudoinia compniacensis is a darkly pigmented microfungus that grows conspicuously on environmental surfaces around warehouses where alcoholic spirits are stored in wooden casks. This fungus has long been ignored because its primary isolation is very difficult. The present study describes a new semiselective isolation medium for this fungus based on the use of ethanol as a sole carbon source and low levels of nitrogen and trace elements.

Baudoinia, a new genus to accommodate Torula compniacensis.

Baudoinia gen. nov. is described to accommodate Torula compniacensis.