Culture of Kenyan Goat () Undifferentiated Spermatogonia in Feeder-Free Conditions.

The undifferentiated spermatogonial population in mammalian testes contains a spermatogonial stem cell (SSC) population that can regenerate continual spermatogenesis following transplantation. This capacity has the potential to be exploited as a surrogate sires breeding tool to achieve widespread dissemination of desirable genetics in livestock production. Because SSCs are relatively rare in testicular tissue, the ability to expand a population would be advantageous to provide large numbers for transplantation into surrogate recipient males.

Comparative Efficiency for in vitro Transfection of Goat Undifferentiated Spermatogonia Using Lipofectamine Reagents and Electroporation.

Introduction: Spermatogonial stem cells (SSC), also referred to as undifferentiated spermatogonia, are the germline stem cells responsible for continuous spermatogenesis throughout a male's life. They are, therefore, an ideal target for gene editing. Previously, SSC from animal testis have been isolated and transplanted to homologous recipients resulting in the successful reestablishment of donor-derived spermatogenesis.

Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review.

Background And Aim: Spermatogonial stem cells (SSCs) have previously been isolated from animals' testes, cultured , and successfully transplanted into compatible recipients. The SSC unique characteristic has potential for exploitation as a reproductive tool and this can be achieved through SSC intratesticular transplantation to surrogate sires. Here, we aimed at comprehensively analyzing published data on maintenance of SSC isolated from the testes of livestock animals and their applications.

Morphometric and stereological methods for quantifying the coarse structural parameters of the ruminal tissues in sheep.

In ruminants, the rumen is the largest and most significant fore-stomach. Stereological analysis of important structural parameters that may be used to assess the functional capacity of the rumen is lacking. In the current investigation, five rams were used to demonstrate the methods for quantifying salient structural parameters related to rumen function.

Gross and histopathologic findings in sheep with plastic bags in the rumen.

The objective of this study was to evaluate the gross and histo-pathological lesions caused by plastic bags in the rumen of sheep. Sixteen (16) castrated, one-year old Dorper sheep were used for the study. The animals were divided into 4 groups each consisting of 4 sheep.