Combined bronchoalveolar-vascular casting of the canine lung.

Canine bronchoalveolar and vascular corrosion casts were prepared using unfixed tissue and Dow-Corning Room Temperature Vulcanizing Silastic 734. The casts were observed using stereo light microscopy and scanning electron microscopy. The casts show the relationship between the vasculature and airway and demonstrate intricate microanatomical details.

Combined vascular-bronchoalveolar casting using formalin-fixed canine lungs and a low viscosity silicone rubber.

Background: Previous work using unfixed or fixed tissues has shown that casts can be made that demonstrate the three-dimensional structure of tissues such as the bronchoalveolar tree or the vasculature. In this report, a new method for creating a vascular-bronchoalveolar cast is described.

Method: Canine lungs were taken from storage in formalin.

Bronchoalveolar casting using formalin-fixed canine lungs and a low viscosity silicone rubber.

A method for creating tough, flexible, bronchoalveolar casts from formalin-fixed canine lung is described. A lung was washed using simple methods and fixed in 10% neutral-buffered formalin. While still wet with formalin, an intact lobe was injected with silicone sealant, Silastic 734 RTV (Room Temperature Vulcanizing), using a caulk gun.

Clinical characteristics of women with chlamydial cervicitis.

Four hundred ninety-three women were examined, had a cervical culture done for Chlamydia trachomatis and completed a questionnaire. Sixty-one, or 12.4%, were positive for Chlamydia on culture.