Effect of 3-trifluoromethyl-alpha-ethylbenzhydrol (flumecinol) on lipid peroxidation in early human placenta.

A study was made of the lipid peroxidation in the microsomal fraction of human placenta tissue in the presence of flumecinol (Zixoryn, Gedeon Richter Pharmaceutical Works, Budapest, Hungary). The tissue samples were obtained from placenta in early pregnancy and also from placenta at term. Up to a concentration of 1 mumolar, flumecinol (3-trifluoromethyl-alpha-ethyl-benzhydrol), as an enzyme inductor, significantly enhanced the rate of lipid peroxidation but above 1 molar it scarcely influenced it.

The modulating effect of estrogens on luteinizing hormone release in complete androgen insensitivity syndrome before and after gonadectomy and cyclic steroid application.

The response of LH release to exogenous estrogens was studied in three patients with androgen insensitivity syndrome (AIS) before and after gonadectomy and a prolonged treatment with a sequential pill. Estrogen provocation tests were performed in which 0.05 mg/kg body weight estradiol benzoate was administered intramuscularly and serum FSH and LH levels were assessed every 12 hours for 96 hours after gonadectomy, three months after a prolonged treatment with a sequential pill (16 cycles: 9 days mestranol 100 micrograms, 12 days mestranol 80 micrograms and chlormadinone acetate 2 mg) and in one patient before gonadectomy.

Determination of sex hormone-binding globulin in human semen by selective ammonium sulphate precipitation.

A simple method was developed to measure the SHBG capacities of human serum, semen and sperm cells. After suitable dilution, disintegration and addition of labelled dihydrotestosterone-1,2-3 H or testosterone-1,2-3 H, the SHBG was precipitated by the addition of saturated ammonium sulphate in a final concentration of 42.3%.