Re-evaluation of the -Glucosyltransferase IroB Illuminates Its Ability to -Glucosylate Non-native Triscatecholate Enterobactin Mimics.

The pathogen-associated -glucosyltransferase IroB is involved in the biosynthesis of salmochelins, -glucosylated derivatives of enterobactin (Ent), which is a triscatecholate siderophore of enteric bacteria including and . Here, we reassess the ability of IroB to -glucosylate non-native triscatecholate mimics of Ent, which may have utility in the design and development of siderophore-based therapeutics and diagnostics. We establish TRENCAM (TC) and MECAM (MC), synthetic Ent analogs with tris(2-aminoethyl)amine- or mesitylene-derived backbones replacing the trilactone core of Ent, respectively, and their monoglucosylated congeners as substrates of IroB.

The impact of zinc pre-exposure on ciprofloxacin resistance development in .

Introduction: Antimicrobial resistance (AMR) is a global health crisis that is predicted to worsen in the coming years. While improper antibiotic usage is an established driver, less is known about the impact of other endogenous and exogeneous environmental factors, such as metals, on AMR. One metal of interest is zinc as it is often used as a supplement for diarrhea treatment prior to antibiotics.

Pilot and feasibility study of dietary composition with elexacaftor-tezacaftor-ivacaftor concentrations in people with cystic fibrosis.

Background: Nutritional support for people with cystic fibrosis (PwCF) after the implementation of novel drug therapies is shifting from managing malnutrition through a high-fat, high-calorie diet to managing emerging incidences of obesity in this population. Additionally, dietary recommendations prescribed with elexacaftor/tezacaftor/ivacaftor (ETI) recommend taking this drug with a fat-containing meal, which is variably interpreted by patients. This pilot and feasibility study was conducted to assess dietary fat intake and body composition on ETI plasma concentrations.

Perceived Biological Bases of Sexual Orientation and Sexual Prejudice: The Moderating Role of Gender and Religious Beliefs.

Prior correlational studies have shown that belief in the biological theory of sexual orientation (BTSO) is associated with more positive attitudes toward homosexuality. However, individuals often interpret scientific evidence in ways that align with their pre-existing beliefs and motivations. This research experimentally investigated whether gender and religiosity moderate heterosexual individuals' responses to scientific evidence either supporting or refuting BTSO.

Herbivory and water availability interact to shape the adaptive landscape in the perennial forb, Boechera stricta.

Abiotic and biotic factors interact to influence phenotypic evolution; however, identifying the causal agents of selection that drive the evolution and expression of traits remains challenging. In a field common garden, we manipulated water availability and herbivore abundance across three years, and evaluated clinal variation in functional traits and phenology, plasticity, local adaptation, and selection using diverse accessions of the perennial forb, Boechera stricta. Consistent with expectations, drought stress exacerbated damage from herbivores.