An innovative intermittent hypoxia model for cell cultures allowing fast Po oscillations with minimal gas consumption.

Performing hypoxia-reoxygenation cycles in cell culture with a cycle duration accurately reflecting what occurs in obstructive sleep apnea (OSA) patients is a difficult but crucial technical challenge. Our goal was to develop a novel device to expose multiple cell culture dishes to intermittent hypoxia (IH) cycles relevant to OSA with limited gas consumption. With gas flows as low as 200 ml/min, our combination of plate holders with gas-permeable cultureware generates rapid normoxia-hypoxia cycles.

[Babesiasis, pediatric malaria: does confusion exist in Africa?].

The authors describe a case of febrile disease acquired by a nursling in Africa: was it induced by Pl. falciparum or by Babesia sp.? In spite of numerous microscopic and serological appraisals from veterinary and medical authorities, it is difficult to state definitively this subject, but it is more probably a babesiosis of canine or rodent origin.