Single nuclei profiling identifies cell specific markers of skeletal muscle aging, frailty, and senescence.

Aging is accompanied by a loss of muscle mass and function, termed sarcopenia, which causes numerous morbidities and economic burdens in human populations. Mechanisms implicated in age-related sarcopenia or frailty include inflammation, muscle stem cell depletion, mitochondrial dysfunction, and loss of motor neurons, but whether there are key drivers of sarcopenia are not yet known. To gain deeper insights into age-related muscle loss, we performed transcriptome profiling on lower limb muscle biopsies from 72 young, elderly, and frail human subjects using bulk RNA-seq ( = 72) and single-nuclei RNA-seq ( = 17).

The Accuracy of Self-Perception of Obesity in a Rural Australian Population: A Cross-Sectional Study.

Introduction: Obesity is a major public health concern. Accurate perception of body weight may be critical to the successful adoption of weight loss behavior. The aim of this study was to determine the accuracy of self-perception of BMI class.

Identification and Management of Children and Adolescents with Obesity Referred to a General Paediatric Outpatient Department.

Aims To identify all children and adolescents with overweight or obesity attending the outpatient department and audit our processes in their identification and management against NICE standards. Methods A retrospective chart review was performed. BMI charts were used to identify children and adolescents with overweight/obesity.

Giving birth in the Murrumbidgee region: A quantitative and qualitative approach to general practice obstetrics in a rural region.

Objective: There is public concern regarding rural workforce shortages and closure of smaller obstetric centres.

Aim: To identify whether safety is a concern for Murrumbidgee hospitals that fit primary medical care models and ascertain general practitioner (GP) obstetricians' perspectives regarding the benefits and challenges to practising in the region.

Design: Mixed-method retrospective analysis of selected outcomes in the NSW Mothers and Babies Reports 2012-2015 and semi-structured interviews with GP obstetricians.

Senolysis induced by 25-hydroxycholesterol targets CRYAB in multiple cell types.

Cellular senescence is a driver of many age-related pathologies. There is an active search for pharmaceuticals termed senolytics that can mitigate or remove senescent cells by targeting genes that promote the survival of senescent cells. We utilized single-cell RNA sequencing to identify CRYAB as a robust senescence-induced gene and potential target for senolysis.