Pericardial bioscaffold coated with ECM gels and urothelial cells for the repair of a rabbit urinary bladder defect.

Repair of damaged or faulty complex modular organs such as the urinary bladder is a current clinical challenge. The design of constructs for reconstructive urological surgery can draw advantage from the bioactivity of natural extracellular matrix (ECM) bioscaffolds, as well as the activity provided by cells seeded into constructs. Considering these benefits, this work compares the performance of pericardial ECM bioscaffolds and constructs seeded with gel-supported urothelial cells in the repair of urinary bladder defects in rabbits.

Self-adjustment of spectral components to object wave field extraction in off-axis digital holography.

In this paper, we introduce a positioning system to self-adjust the spectral components of holograms recorded by an off-axis digital holography setup. The incidence angle of the reference arm is carried out by two motorized actuators controlled by an algorithm that automatically adjusts it to avoid the overlapping of the spectral components. The right positioning of the spectral components allows selection of a spectral region to extract only the virtual component so that, after an inverse Fourier transformation, the object wave field can be obtained, thus eliminating the undesired components and increasing the image quality of the reconstructed image.

Imaging of bee honey sugar crystals by second-harmonic generation microscopy.

Bee honey is an exceptionally nutritious food with unique chemical and mineral contents. This report introduces the use of the second-harmonic generation (SHG) microscopy for imaging honey sugar crystals' morphology as an alternative for its authentication process. The crystals and their boundaries are clearly observed with SHG compared with bright-field microscopy, where the liquid honey avoids the visualization of a sharp image.

Measurement of morphology thickness and refractive index in a melanoma A375 cell line using digital holographic microscopy.

Digital holographic microscopy (DHM) is a technique that has high potential for analyzing biological samples and has been successfully applied to the study of cells and cell lines providing information about important parameters such as refractive index, morphology, and dry mass, among others; it has also found applicability to study the effects of therapeutic treatments. Finding the size and shape of cells is important since they tend to change in the presence of some pathologies. In this research work, we obtain the morphology thickness and refractive index of the A375 melanoma cell line through a slight tilting of the cell in a DHM setup.

Object wave field extraction in off-axis holography by clipping its frequency components.

Suitable extraction of an object's wave field in an off-axis digital holographic array is the core for any numerical reconstruction process. In this manuscript, we propose an algorithm that reduces the number of pixels needed for an object's wave field trimming, reducing notably its processing time without significant changes in the quality of the reconstructed image. Our method generates an extraction window containing a complete object's wave field.