Dopamine injections to the midbrain periaqueductal gray inhibit vocal-motor production in a teleost fish.

Across vertebrates, the midbrain periaqueductal gray (PAG) plays a critical role in social and vocal behavior. Dopaminergic neurotransmission also modulates these behaviors, and dopaminergic innervation of the PAG has been well documented. Nonetheless, the potential role of dopamine in shaping vocal production at the level of the PAG is not well understood.

Ca entry units in a superfast fish muscle.

Over the past two decades, mounting evidence has demonstrated that a mechanism known as store-operated Ca entry (SOCE) plays a crucial role in sustaining skeletal muscle contractility by facilitating Ca influx from the extracellular space during sarcoplasmic reticulum (SR) Ca depletion. We recently demonstrated that, in exercised fast-twitch muscle from mice, the incidence of Ca entry units (CEUs), newly described intracellular junctions between dead-end longitudinal transverse tubular (T-tubule) extensions and stacks of sarcoplasmic reticulum (SR) flat cisternae, strictly correlate with both the capability of fibers to maintain contractions during fatigue and enhanced Ca influx SOCE. Here, we tested the broader relevance of this result across vertebrates by searching for the presence of CEUs in the vocal muscles of a teleost fish adapted for extended, high-frequency activity.

A simple and robust LC-ESI single quadrupole MS-based method to analyze neonicotinoids in honey bee extracts.

Over the past years, neonicotinoids such as thiacloprid and flupyradifurone have gained considerable scientific and public interest. These molecules used as active compounds in pesticides are known due to cause drastic negative long-time effects on pollinators and even human health. Therefore, determining trace amounts of neonicotinoid in different environmental matrices by liquid chromatography coupled with mass selective detectors (LC-MS/MS or LC-Q-TOF/MS) has become an important methodology.

Sexually-dimorphic expression of tyrosine hydroxylase immunoreactivity in the brain of a vocal teleost fish (Porichthys notatus).

Vocal communication has emerged as a powerful model for the study of neural mechanisms of social behavior. Modulatory neurochemicals postulated to play a central role in social behavior, related to motivation, arousal, incentive and reward, include the catecholamines, particularly dopamine and noradrenaline. Many questions remain regarding the functional mechanisms by which these modulators interact with sensory and motor systems.

Vocal-motor and auditory connectivity of the midbrain periaqueductal gray in a teleost fish.

The midbrain periaqueductal gray (PAG) plays a central role in the descending control of vocalization across vertebrates. The PAG has also been implicated in auditory-vocal integration, although its precise role in such integration remains largely unexplored. Courtship and territorial interactions in plainfin midshipman fish depend on vocal communication, and the PAG is a central component of the midshipman vocal-motor system.