Nonopsonic phagocytosis and intracellular killing of Salmonella typhimurium by peritoneal macrophages of mice immunized with slime-extract from Pseudomonas aeruginosa.

Treatment of mice with slime-extract (SE) from Pseudomonas aeruginosa strain 219 failed to increase the proportion of ingested cells of Salmonella typhimurium by immune macrophages (M phi) of mice. However, activated with SE peritoneal SE peritoneal M phi displayed the enhancement of their nonspecific bactericidal activity. It has been found that immune M phi, depending on the dose of SE used for vaccination, killed intracellularly 38 to 67% of bacterial cells, whereas the mean value for the bactericidal capacity of normal resident phagocytes was 7%.

Depression by slime-extract from Pseudomonas aeruginosa of delayed type hypersensitivity to sheep erythrocytes.

High doses of slime-extract from Pseudomonas aeruginosa was found to suppress the delayed type hypersensitivity response to sheep erythrocytes when administered intraperitoneally 1 to 3 days before or 3 days after intravenous sensitization of mice. Moreover, the spleen cells from sensitized and slime-extract treated mice transferred the depression to normal recipients. Inhibition of DTH response was also seen when recipients were injected with slime-extract 24 h before they were infused with spleen cells from donor mice immunized with sheep erythrocytes.

Phagocytosis and intracellular killing of Pseudomonas aeruginosa by peritoneal macrophages of mice.

The poorly mucoid P. aeruginosa 87 strain was better phagocytized by peritoneal macrophages from normal mice than highly mucoid P. aeruginosa 219.

Phagocytosis and intracellular killing of mucoid and nonmucoid variants of Pseudomonas aeruginosa by polymorphonuclear leukocytes: effect of specific immune sera.

Nonmucoid variants (NM) of 6 Pseudomonas aeruginosa strains were better phagocytized and intracellulary killed by rabbit peripheral polymorphonuclear leukocytes (PMNs) than their mucoid variants (M). The ratios of ingested and killed bacteria of both variants significantly increased in the presence of immune serum with antibodies against slime and somatic antigen (anti-OM) in the phagocytic mixture. Immune sera prepared for slime layer only enhanced the both activities of PMNs against M variant.

Kinetics of humoral response and mechanism of the protection in mice vaccinated with slime-extract from Pseudomonas aeruginosa.

The in vivo experiments on the immunity of mice to P. aeruginosa showed that the vaccination with slime-extract or transfer of immune anti-slime serum enhances the resistance of mice to these organisms. Immunity against P.