Involvement of treponemal surface-located protein and carbohydrate moieties in the attachment of Treponema denticola ATCC 33520 to cultured rat palatal epithelial cells.

We studied the nature of attachment of Treponema denticola ATCC 33520 to a microscopically distinct population of rounded rat palatal epithelial cells. The motility of the freshly harvested spirochetes appeared not be a prerequisite for attachment. Treatment of T.

Attachment of T. denticola strains ATCC 33520, ATCC 35405, B11 and Ny541 to a morphologically distinct population of rat palatal epithelial cells.

In the present study an assay for the attachment of T. denticola to epithelial cells is described. An indirect immunohistochemical staining method, using two native polyclonal antisera, revealed dark-brown coloured spirochetes attached to rat palatal epithelial cell (RPE) monolayers.

Attachment of Treponema denticola strains to monolayers of epithelial cells of different origin.

The attachment of 10 different Treponema denticola strains to monolayers of 4 types of epithelial cells derived from rat palatal epithelium, guinea pig ear, human buccal epithelium and human corneal epithelium was screened microscopically. Most T. denticola strains were able to attach to all four types of epithelial cells.

Adherence of oral treponemes and their effect on morphological damage and detachment of epithelial cells in vitro.

The present study shows adherence of Treponema denticola L12D and Treponema vincentii RitzA to guinea pig ear epithelial cells in vitro. The number of adhering organisms was positively related with the treponemal concentration and contact time. Incubation of washed T.

Ultrastructural study of epithelial cell attachment to implant materials.

Attachment of gingival tissues to the protruding post of an endosseous dental implant is of great importance for the prognosis of its clinical success. Little is known about the requirements an implant material must meet to secure a durable permucosal seal. The objective of this study, therefore, was to gain insight into the morphology of epithelial cell-implant interfaces for a number of well-known or potential implant materials.