Male rats were deprived of water for 5 days, and then given water ad libitum for 3, 7, 10 or 14 days. Plasma osmolarity returned to normal in less than 3 days, while pituitary vasopressin (AVP) and oxytocin (OXT) only returned to control levels after 14 days. Sections of the supraoptic nucleus (SON) were hybridized with 35S-labelled cDNA (OXT) or oligonucleotide (AVP) probes.
View Article and Find Full Text PDFInfection of Vero (monkey) cells by Germiston bunyavirus is highly cytopathic with cell lysis and virus production at a high titre, whereas infection of Aedes albopictus C6/36 (mosquito) cells leads, after an acute primary phase, to a persistent non-cytopathic infection with a loss in virus production. In this report we demonstrate that single-stranded RNA probes can be successfully used in an in situ hybridization assay to quantify viral expression during this persistent infection. The steady-state levels of viral S-RNA segment (genomic and messenger sense) during the acute phase were similar to those observed in lytically infected Vero cells, but appeared delayed.
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