Publications by authors named "J M Sanchez Calvo"
Background And Objectives: Highly effective procedures for the preparation of allogeneic platelet gel (PG) use Ca-gluconate and batroxobin, an expensive commercial reagent. In this preliminary study, we explored the use of the plasmin-inhibitor, low-cost drug tranexamic acid (TXA) in place of batroxobin, based on the literature supporting TXA ability to prevent fibrinolysis and stabilize the gel formed by fibrin polymerization prompted by Ca-gluconate.
Materials And Methods: Eight blood centres determined PG weight and volume of non-gelled, liquid portion in 116 PG prepared in 20-mL commercial BioNest D bags.
The objective of the present study was to determine the variability of platelet lysate-derived extracellular vesicles (pEV), in terms of characteristics and functionality through wound healing assays, when isolated either from platelet concentrates (PC, obtained from 5 donors) or from multiple PC (MPC, that is 50 donors). pEV were isolated under GMP-like conditions in a clean room using Size Exclusion Chromatography (SEC). The differential characteristics between pEV obtained from PC (PC-EV) or MPC (MPC-EV) were evaluated by means of protein concentration, Nanoparticle Tracking Analysis (NTA), Transmission Electron Microscopy (TEM), and flow cytometry using the MACSPlex™ arrays for surface analysis profiling of EV.
View Article and Find Full Text PDFPurpose: This study aims to culturally adapt and validate the Stroke Upper Limb Capacity Scale (SULCS) and Action Research Arm Test (ARAT) for use with post-stroke patients in Italy.
Methods: The original scales were translated and culturally adapted following the "Translation and Cultural Adaptation of Patient Reported Outcomes Measures - Principles of Good Practice" guidelines. We assessed internal consistency and test-retest reliability, while concurrent validity was evaluated using Pearson correlation coefficients with the Italian version of the Disability Arm Shoulder and Hand (DASH).
Article Synopsis
- * Research using single-cell RNA sequencing identified a specific group of chemotherapy-resistant leukemic cells (CLCs) that are quiescent and marked by high CD44 expression, allowing them to evade treatment.
- * The study found that these CLCs exhibit unique gene activity and enhanced E-selectin binding during relapse, suggesting potential pathways for improving prognosis and developing new therapies.
Article Synopsis
- * In a study of nestling eagle owls, ARs were found in 91.5% of blood samples, with many individuals showing multiple AR compounds, primarily second-generation ARs (SGARs).
- * While the overall health of the sampled owls appeared good, there was a positive but not significant correlation between AR levels and prothrombin time; higher AR concentrations were linked to urban areas and landfills, indicating a need for better monitoring of these pollutants.