Publications by authors named "J M S Grimsley"
Article Synopsis
- Wastewater-based epidemiology (WBE) is a valuable tool for monitoring community infections, including silent cases of SARS-CoV-2 and other pathogens.
- Next-generation sequencing (NGS) allows for more detailed analyses of viruses, but many facilities still don't use these advanced methods in their routine surveillance.
- The review discusses how to better incorporate NGS into WBE programs to improve detection of emerging viruses and enhance global health response strategies.
Article Synopsis
- * The study examined how well different types of wastewater samples preserve the genetic material of various viruses under various storage conditions, finding that certain genes, like the Spike (S) gene of SARS-CoV-2, are less stable during freeze-thaw cycles compared to others.
- * Results indicated that storing raw wastewater samples at 4 °C optimally preserves SARS-CoV-2, while a laboratory comparison showed that Phi6 phage serves well as a stable surrogate for studying SARS-CoV-2, with minimal degradation
Wastewater-based epidemiology is now widely used in many countries for the routine monitoring of SARS-CoV-2 and other viruses at a community level. However, efficient sample processing technologies are still under investigation. In this study, we compared the performance of the novel Nanotrap® Microbiome Particles (NMP) concentration method to the commonly used polyethylene glycol (PEG) precipitation method for concentrating viruses from wastewater and their subsequent quantification and sequencing.
View Article and Find Full Text PDFWastewater-based epidemiology (WBE) has proven to be a powerful tool for the population-level monitoring of pathogens, particularly severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For assessment, several wastewater sampling regimes and methods of viral concentration have been investigated, mainly targeting SARS-CoV-2. However, the use of passive samplers in near-source environments for a range of viruses in wastewater is still under-investigated.
View Article and Find Full Text PDFDiurnal changes in pathogenic and indicator virus concentrations in wastewater.
Environ Sci Pollut Res Int
December 2023
Wastewater-based epidemiology (WBE) has been commonly used for monitoring SARS-CoV-2 outbreaks. As sampling times and methods (i.e.
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