How to Interpret a Positive PCR Result Using the BD MAX System in the Absence of Positive Culture?

The aim of this study was to evaluate, using two independent polymerase chain reaction (PCR) formats, the results of detection by the BD MAX Enteric Bacterial Panel PCR (Becton Dickinson, Le Pont de Claix, France) in the absence of positive culture. A total of 77 samples found positive for on BD MAX but negative by culture were studied. Upon reception, one in-house real-time-PCR for and a PCR with the RIDAGENE Bacterial Stool Panel (r-biopharm, Darmstadt, Germany) were performed.

Dialister pneumosintes associated with human brain abscesses.

In this report, we review two cases of brain infection due to Dialister pneumosintes in previously healthy patients. The bacterium was isolated from the first patient by blood culture and directly from a brain abscess in the second patient. In both cases, the infection was suspected to be of nasopharyngeal or dental origin.