Evaluation of angiogenesis in delayed skin flaps using a monoclonal antibody for the vascular endothelium.

The angiogenic process in delayed and undelayed flaps was evaluated in relation to different periods of delay (6 h, 72 h and 7 days) and at different times after the definitive elevation of the flaps (48 h, 96 h and 7 days). An immunohistochemical method has been tested to stain the endothelial cells of capillaries and venules, providing a high specificity for labelling these vessels. No signs of angiogenesis were found in undelayed and 6-h delayed flaps at 48 h but there was an increase of capillaries from 48 h to 7 days after elevation of the flaps.

Restrictive cardiomyopathy caused by chloroquine.

A 59 year old white woman who had been treated with chloroquine phosphate for 25 years presented with signs of congestive heart failure and was diagnosed as having restrictive cardiomyopathy by non-invasive methods. Electron microscopy of a biopsy specimen of skeletal muscle showed lesions compatible with chloroquine myopathy. The patient died five weeks after presentation.

Electrocardiographic patterns in V4R lead in experimental right ventricular infarction of rats.

Twenty-four male rats that became hypertensive after complete ligature of the abdominal aorta, just above the origin of the left renal artery, showed combined myocardial infarction. To study the electrocardiographic patterns of the experimental right ventricular infarction, the V4R right thoracic lead was recorded at different time intervals after aortic ligature. The patterns recorded were as follows: Q waves in 23 cases (95.

Morphological changes in the vascularisation of delayed flaps in rabbits.

The effect of the delay phenomenon on the vascularisation of the pectoralis major myocutaneous flap was studied in 32 rabbits. In 36 flaps, submitted to different methods of delay, vascularisation was studied by making the tissue transparent and comparing the results with a control group of 16 undelayed flaps. In another 12 flaps, 6 of them previously delayed, the vascularisation of the skin was studied by light microscopy and through a morphometric analysis (image digitalizing process).

[Biochemical plasma changes in essential hypertension patients treated for a year].

The possibility that certain antihypertensive drugs produce metabolic alterations in patients with hypertension treated during one year, has been prospectively assessed in 40 patients with essential hypertension with matched age and blood pressure readings. Four groups (Gr) of 10 patients each were studied. Group 1 received 100 mg/day of atenolol (AT), group 2 received 5 mg of amiloride and 50 mg of hydrochlorothiazide (4 tablets per week), group 3 received 40 mg/day of nifedipine retard (NF) and group 4 received 50 mg/day of captopril (CP).