Effect of transgene on salt tolerance of tobacco.

To explore the effects of salt-tolerance gene accumulation on salt tolerance in transgenic plant, we used four types of plant expression vector (N27, N28, N29, and N30) carrying mtlD, mtlD + gutD, mtlD + gutD + BADH, mtlD + gutD + BADH + sacB genes respectively, to transform tobacco through Agrobacterium-mediated method. Transgenic lines were identified through polymerase chain reaction (PCR) detection. Transgenic lines and non-transgenic plant (CK) were subjected to 6‰ sodium chloride solution stress; then, fluorescence quantitative PCR (FQ-PCR) and salt tolerance indexes were used to assess characteristics.

Machine learning based models for predicting presentation delay risk among gastric cancer patients.

Objective: Presentation delay of cancer patients prevents the patient from timely diagnosis and treatment leading to poor prognosis. Predicting the risk of presentation delay is crucial to improve the treatment outcomes. This study aimed to develop and validate prediction models of presentation delay risk in gastric cancer patients by using various machine learning models.

Global insights into aging: a multidisciplinary approach to understanding and addressing age-related challenges.

Aging has ascended to the forefront of scientific exploration, demanding a concerted global focus. The 2024 China Aging Science Conference and International Conference on Aging Biology hosted a panel discussion that brought international experts to delve into the complexities of aging research. The discussion underscores the imperative need for a multidisciplinary approach, integrating reductionist and holistic perspectives to unravel the molecular and epigenetic underpinnings of the aging process.

Safe distances for transforaminal posterior lumbar interbody fusion under one-hole split endoscopy: three-dimensional reconstruction measurement of 1-degree degenerative lumbar spondylolisthesis at the L segment.

Background: One-hole split endoscopy (OSE) is a novel endoscopic technique that offers some advantages in spinal surgery. However, without a clear understanding of the safe zone for OSE, surgeons risk injuring nerve roots during the procedure. This study aimed to measure the safe distances among critical bone markers, the intervertebral space and nerve roots between 1-degree degenerative lumbar spondylolisthesis (DLS) and non-DLS at the L segment in patients via three-dimensional reconstruction and to compare the differences in relevant safety distances between the two groups.

Sulfate-reducing bacteria block cadmium and lead uptake in rice by regulating sulfur metabolism.

Aim: This study was dedicated to investigating the role of sulfur metabolic processes in sulfate-reducing bacteria in plant resistance to heavy metal contamination.

Methods And Results: We constructed sulfate-reducing bacterial communities based on the functional properties of sulfate-reducing strains, and then screened out the most effective sulfate-reducing bacterial community SYN1, that prevented Cd and Pb uptake in rice through hydroponic experiment. This community lowered Cd levels in the roots and upper roots by 36.