Publications by authors named "J M Poirier"
The precuneus is a site of early amyloid-beta (Aβ) accumulation. Previous cross-sectional studies reported increased precuneus fMRI activity in older adults with mild cognitive deficits or elevated Aβ. However, longitudinal studies in early Alzheimer's disease (AD) are lacking and the relationship to the Apolipoprotein-E () genotype is unclear.
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- - APOB, a protein found in cholesterol-rich lipoproteins, is connected to Alzheimer's disease (AD), with high levels of APOB-containing LDL linked to both early-onset and late-onset forms of the disease.
- - Research conducted across four cohorts demonstrated that while brain APOB protein levels positively correlated with AD-related pathology and cognitive decline, APOB mRNA levels showed an inverse relationship.
- - The study highlights the possible implications of APOB in AD, revealing associations with Tau pathology in early stages and Aβ deposition in advanced stages, suggesting its role in lipoprotein metabolism impacts AD progression.
The cotranslational misfolding of the cystic fibrosis transmembrane conductance regulator chloride channel (CFTR) plays a central role in the molecular basis of CF. The misfolding of the most common CF variant (ΔF508) remodels both the translational regulation and quality control of CFTR. Nevertheless, it is unclear how the misassembly of the nascent polypeptide may directly influence the activity of the translation machinery.
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- Interferons (IFNs) are important for how our bodies respond to viruses, and this study used CRISPR to find human genes affecting SARS-CoV-2 infection with and without IFN.
- The research identified 28 key genes linked to COVID-19, especially those involving the IFN pathway, including PLSCR1, which can limit virus entry without needing IFN.
- PLSCR1’s ability to block SARS-CoV-2 was reduced by the overexpression of TMPRSS2, and some virus variants have evolved to bypass PLSCR1’s defense, suggesting ongoing challenges in controlling the virus.
Retrons are a retroelement class found in diverse prokaryotes that can be adapted to augment CRISPR-Cas9 genome engineering technology to efficiently rewrite short stretches of genetic information in bacteria and yeast; however, efficiency in human cells has been limited by unknown factors. We identified non-coding RNA (ncRNA) instability and impaired Cas9 activity as major contributors to poor retron editor efficiency. We re-engineered the Eco1 ncRNA to incorporate an exoribonuclease-resistant RNA pseudoknot from the Zika virus 3' UTR and devised an RNA processing strategy using Csy4 ribonuclease to liberate the sgRNA and ncRNA.
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