Publications by authors named "J M Munoz Camarena"
A multitude of tools now exist that allow us to precisely manipulate the human genome in a myriad of different ways. However, successful delivery of these tools to the cells of human patients remains a major barrier to their clinical implementation. Here we introduce a new cellular approach for genetic engineering, ecreted article nformation ransfer (SPIT) that utilizes human cells as delivery vectors for genetic engineering.
View Article and Find Full Text PDFSome gene polymorphisms can lead to monogenic diseases, whereas other polymorphisms may confer beneficial traits. A well-characterized example is congenital erythrocytosis-the non-pathogenic hyper-production of red blood cells-that is caused by a truncated erythropoietin receptor. Here we show that Cas9-mediated genome editing in CD34 human haematopoietic stem and progenitor cells (HSPCs) can recreate the truncated form of the erythropoietin receptor, leading to substantial increases in erythropoietic output.
View Article and Find Full Text PDFArticle Synopsis
- Over 38 million people worldwide live with HIV and AIDS, highlighting the need for better understanding of engagement in HIV prevention and care services as new options become available.
- This systematic review focuses on preferences of individuals living with or at risk of HIV, analyzing studies conducted from January 2018 to October 2022 to identify factors influencing their willingness to use these services.
- The review will compile data from various databases, with findings intended for presentation at conferences and publication in a peer-reviewed journal, as no ethical review is needed due to the use of publicly available data.
A multitude of tools now exist that allow us to precisely manipulate the human genome in a myriad of different ways. However, successful delivery of these tools to the cells of human patients remains a major barrier to their clinical implementation. Here we introduce a new cellular approach for genetic engineering, ecreted article nformation ransfer (SPIT) that utilizes human cells as delivery vectors for genetic engineering.
View Article and Find Full Text PDFRole of the first WHO mutation catalogue in the diagnosis of antibiotic resistance in Mycobacterium tuberculosis in the Valencia Region, Spain: a retrospective genomic analysis.
Lancet Microbe
January 2024
Article Synopsis
- In June 2021, the WHO released a comprehensive catalogue of mutations in Mycobacterium tuberculosis that are linked to drug resistance, prompting researchers to evaluate its effectiveness for diagnosing drug-resistant tuberculosis in the relatively low prevalence area of Valencia, Spain.
- A retrospective genomic study analyzed 785 tuberculosis isolates collected between 2014-2016, utilizing whole-genome sequencing (WGS) to predict resistance profiles based on the catalogue and comparing these predictions with actual phenotypic results.
- The study found that while sensitivity for predicting resistance varied, with the highest at 85.4% for isoniazid, overall pan-susceptibility accuracy was 96.4%, highlighting some discrepancies in certain isolates that carried mutations known to cause borderline