Apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods.

This paper presents results from a study to develop cryopreservation procedures for apices of several strawberry genotypes. Five Fragaria x ananassa Duch. cultivars and two wild species (F.

Chemical Alternatives to Methyl Bromide in Spanish Strawberry Nurseries.

Strawberry runners are a high-value cash crop in Spain that requires vigorous transplants free of pathogens. Preplant soil fumigation with methyl bromide, or with mixtures of methyl bromide and chloropicrin, is a standard practice for controlling soilborne diseases. Soil fumigants chloropicrin, 1,3-dichloropropene, dazomet, metam-sodium, metam potassium, and dimethyl disulfide were evaluated in combination with different plastic films as alternatives for methyl bromide soil fumigation of strawberry nurseries.

Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene.

Strawberry (Fragaria x ananassa, Duch., cv Chandler) is a soft fruit with a short postharvest life, mainly due to a rapid lost of firm texture. To control the strawberry fruit softening, we obtained transgenic plants that incorporate an antisense sequence of a strawberry pectate lyase gene under the control of the 35S promoter.

Shoot regeneration and Agrobacterium-mediated transformation of Fragaria vesca L.

An efficient and reliable method for shoot regeneration from leaf disks of Fragaria vesca L. has been developed. This protocol has been successfully employed to obtain transformed plants using Agrobacterium tumefaciens as gene vector.