Altered Protein Structures and Neoepitopes in Lupus Neutrophils From Dysregulated Splicing of Messenger RNA.

Objective: To test whether messenger RNA (mRNA) splicing is altered in neutrophils from patients with systemic lupus erythematosus (SLE) and can produce neoantigens.

Methods: RNA sequencing of neutrophils from patients with SLE (n = 15) and healthy donors (n = 12) were analyzed for mRNA splicing using the RiboSplitter pipeline, an event-focused tool based on SplAdder with subsequent translation and protein domain annotation. RNA sequencing from SARS-CoV2-infected individuals was used as an additional comparator.

Test Validity of a Single-Item Food Insecurity Screening Assessment Among College Students.

Purpose: Given the high prevalence of food insecurity among college students, there is an interest in identifying whether the use of a single item can adequately screen for food insecurity. The current study aimed to determine the validity of a single-item food insecurity screening question among college students.

Design: Cross-sectional study exploring food insecurity among racial and ethnic undergraduate college students.