Renal responses during a dry saturation dive to 450 msw.

Four subjects were compressed to a simulated depth of 450 msw (46 bar) for 37 days in the main research chamber of the German underwater simulator diving facility at the GKSS Research Center, Geesthacht. The ambient gas was trimix. Urine was collected at 0700, 1300, and 1900 h each day for analysis of Na+, K+, volume, osmolality, and creatinine.

Urinary vasopressin and aldosterone and plasma volume during a saturation dive to 450 m.

Urinary vasopressin (VP), aldosterone (ALDO), osmotic substances, sodium excretion, and plasma volume were assessed in 4 healthy male divers during 2 predive control days, 2 compression days, 6 days at 46 atm abs, and 26 days of decompression with stops at 37 and 27 atm abs. At pressure the ambient gas was trimix (0.5 atm abs O2:5% N2:remainder He).

Effects of methylguanidine on sodium and organic ion transport.

We studied the acute effect of methylguanidine (MG), a suspected uremic toxin that accumulates in renal failure, on p-aminohippurate (PAH) and tetraethylammonium (TEA) uptake in rabbit kidney slices, on Na+,K+ ATPase activity in the microsomal fraction of rabbit kidneys, and on transepithelial active Na transport across toad skin. MG at concentrations ranging from 0.05 (similar to that reported in uremic patients) to 1.

Interaction of heptanol and pressure on sodium and chloride transport by toad skin.

We examined the interaction of heptanol and hydrostatic pressure on Na+ and Cl- transport in isolated toad skin. In the presence of Cl-, heptanol decreased short-circuit current (Isc) and total transepithelial resistance (Rt). However, in the absence of Cl- in the mucosal bath, heptanol increased Rt, although it retained the same inhibitory effect on Isc.

Persistent proximal tubule dysfunction late in Heymann nephritis.

To determine whether proximal tubule function returned to normal after cessation of active immunological injury in Heymann nephritis, we compared kidney function in an acute stage of the disease, when antibodies were being deposited on the brush border, to a later, chronic stage. Renal blood flow measurements via a flow probe, along with clearance and micropuncture techniques, were used to measure renal plasma flow, glomerular filtration rate, protein and albumin excretion, organic ion (PAH and TEA) extraction and tubule fluid inulin concentration. Proximal tubule fluid reabsorption, which was depressed in the acute stage of injury, returned to normal in chronic Heymann nephritis, but both PAH and TEA extraction continued to be depressed.