T Cell-Specific Inactivation of the PI3K p110α Catalytic Subunit: Effect in T Cell Differentiation and Antigen-Specific Responses.

Class IA PI3K p110δ and p110α subunits participate in TCR and costimulatory receptor signals involved in T cell-mediated immunity, but the role of p110α is not completely understood. Here, we analyzed a mouse model of the Cre-dependent functional inactivation of p110α (kinase dead) in T lymphocytes (p110αKD-T, KD). KD mice showed increased cellularity in thymus and spleen and altered T cell differentiation with increased number of CD4CD8 DP thymocytes, enhanced proportion of CD4 SP lymphocytes linked to altered apoptosis, lower Treg cells, and increased AKT and ERK phosphorylation in activated thymocytes.

Effectiveness of an embedded oral health educational intervention in an undergraduate nursing program: A mixed methods study.

Aim: This study aims to evaluate the effectiveness of an oral health educational intervention designed for undergraduate nursing students.

Background: The role of oral health promotion and screening has been extended to non-dental health professionals, for example, nurses. To prepare the future workforce for these responsibilities, an educational intervention underpinned by Mezirow's Transformative Learning Theory was codesigned and implemented in an Australian undergraduate nursing program.

Seroprevalence of Naegleria fowleri in the Houston-Galveston Texas Population.

Naegleria fowleri is a free-living amoeba and the causative agent of Primary Amebic Meningoencephalitis (PAM), a rare yet almost always fatal disease that primarily affects children. While only 431 PAM cases have been reported worldwide, the southern states of the United States, including Texas, report the highest number of cases. Despite the high mortality of the disease, studies have identified antibodies to N.

Enhancing support for patients with low anterior resection syndrome: insights and educational resources from the LARSCAT project.

Purpose: To address the educational gaps and support needs of patients with low anterior resection syndrome (LARS) following rectal cancer surgery. The LARSCAT project aimed to develop comprehensive resources to better inform patients and enhance their quality of life.

Methods: A qualitative study was conducted between November 2022 and March 2023.

Microfluidics-Based Ionic Catch and Release Oligosaccharide Synthesis (ICROS-Microflow) to Expedite Glycosylation Chemistry.

A continuous microfluidic glycosylation strategy that requires no chromatography between steps and significantly expedites glycosylation chemistry is described. This practical approach incorporates the advantages of imidazolium-based chromatography-free purification and in situ mass spectrometry reaction monitoring to achieve fast reaction optimization and shorter reaction times. We demonstrate the utility of this strategy in the synthesis of a series of glycoside targets, including an α-(1,6)-trimannoside and a branched Man oligomannoside, within 1 day.