Publications by authors named "J M Camadro"
Oxidative stress is a biological principle affecting all life on Earth and is also an important factor in the pathogen-host relationship. The pathogenic free-living amoeba has several pathways to cope with reactive oxygen species and the damage that they cause. In this study, we aimed to provide a comprehensive analysis of the amoeba's response to different sources of oxidative stress.
View Article and Find Full Text PDFCorrigendum to Multiomic analysis in fibroblasts of patients with inborn errors of cobalamin metabolism reveals concordance with clinical and metabolic variability [eBioMedicine 99 (2024), 104911].
EBioMedicine
December 2024
Article Synopsis
- - The study investigates the complex clinical and metabolic effects associated with inborn errors of cobalamin metabolism, particularly focusing on cblC and epi-cblC cases, to better understand their variability and underlying mechanisms.
- - Researchers utilized metabolomic, genomic, proteomic, and post-translational modification analyses on fibroblasts from cblC and cblG patient cases, revealing notable disruptions in metabolic pathways such as the urea cycle and mitochondrial energy production.
- - Findings highlight significant changes in enzyme expression and activity, which could explain clinical symptoms like neurological issues and developmental delays in patients; further research is needed to confirm these connections.
Article Synopsis
- Down syndrome is a common chromosomal disorder associated with blood issues, particularly mild to moderate thrombocytopenia (low platelet count), which doesn't typically lead to bleeding problems.
- Researchers studied the effects of Dyrk1A overexpression in mice and found it resulted in a 20% decrease in platelet count and a surprising 50% reduction in bleeding time.
- The study revealed that the changes were linked to increased levels of fibronectin and fibrinogen in the plasma and liver, suggesting Dyrk1A has a new role in promoting these proteins, which may explain the observed discrepancies in bleeding tendencies.
The simple light isotope metabolic-labeling technique relies on the biosynthesis of amino acids from U-[C]-labeled molecules provided as the sole carbon source. The incorporation of the resulting U-[C]-amino acids into proteins presents several key advantages for mass-spectrometry-based proteomics analysis, as it results in more intense monoisotopic ions, with a better signal-to-noise ratio in bottom-up analysis. In our initial studies, we developed the simple light isotope metabolic (SLIM)-labeling strategy using prototrophic eukaryotic microorganisms, the yeasts and , as well as strains with genetic markers that lead to amino-acid auxotrophy.
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