Detection of simple mutations and polymorphisms in large genomic regions.

We have developed a novel technology that makes it possible to detect simple nucleotide polymorphisms directly within a sample of total genomic DNA. It allows, in a single Southern blot experiment, the determination of sequence identity of genomic regions with a combined length of hundreds of kilobases. This technology does not require PCR amplification of the target DNA regions, but exploits preparative size-fractionation of restriction-digested genomic DNA and a newly discovered property of the mismatch-specific endonuclease CEL I to cleave heteroduplex DNA with a very high specificity and sensitivity.

Geriatric evaluation is a useful adjunct to traditional medical care of the frail older patient.

GEMU evaluation is most helpful in complicated cases where frail elderly patients appear likely to require custodial nursing home care in the near future. The best results and most significant reductions in subsequent mortality have occurred in less ill or more recently debilitated elders with a reasonable chance of returning to modified self-care in the home or in assisted living situations. Adapting these principles in an effective, cost-efficient manner for outpatient care is the focus of the majority of current investigations of CGA/GEM.

Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family.

Mutants of Pseudomonas aeruginosa PAO1 that were deficient in the ability to produce proteases that degrade casein were detected among the survivors of chemical mutagenesis. One such mutant (PDO31) showed reduced production of elastolytic activity, beta-hemolytic activity, and pyocyanin. A 4.

Appropriate and inappropriate prescribing of narcotics for ambulatory HIV-positive patients.

Objective: To assess the appropriateness of narcotic-prescribing practices in an ambulatory clinic for patients infected with HIV.

Design, Setting, And Patients: The medical records of 220 (190 HIV-positive) patients, seen in a clinic primarily designed for the long-term follow-up of ambulatory HIV-infected patients and located in an inner-city, public teaching hospital, were retrospectively reviewed to determine the prevalence and appropriateness of prescribing Drug Enforcement Administration schedule 2 narcotics. Appropriateness was based on published guidelines for the use of narcotics in the treatment of cancer patients.