A Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of in the Field.

A real-time loop-mediated isothermal amplification (LAMP) assay for the detection of the causal pathogen of lettuce downy mildew, was developed and validated to aid in-field detection of airborne inoculum. Assay specificity was confirmed against a range of other pathogenic oomycete and fungal spp., and sensitivity of the assay for the detection of DNA extracted from sporangia was evaluated.

Real-Time PCR and LAMP Assays for the Detection of Spores of and Sporangia of to Inform Disease Risk Forecasting.

Real-time loop-mediated isothermal amplification (LAMP) assays for the detection of sporangia of the causal pathogen of late blight, , and spores of the main causal pathogen of early blight, , were developed to facilitate the in-field detection of airborne inoculum to improve disease forecasting. These assays were compared with an existing real-time PCR assay for and a newly developed real-time PCR assay for . Primers were designed for real-time LAMP of and .

Crop connectivity under climate change: future environmental and geographic risks of potato late blight in Scotland.

The impact of climate change on dispersal processes is largely ignored in risk assessments for crop diseases, as inoculum is generally assumed to be ubiquitous and nonlimiting. We suggest that consideration of the impact of climate change on the connectivity of crops for inoculum transmission may provide additional explanatory and predictive power in disease risk assessments, leading to improved recommendations for agricultural adaptation to climate change. In this study, a crop-growth model was combined with aerobiological models and a newly developed infection risk model to provide a framework for quantifying the impact of future climates on the risk of disease occurrence and spread.

Utilizing "Omic" Technologies to Identify and Prioritize Novel Sources of Resistance to the Oomycete Pathogen Phytophthora infestans in Potato Germplasm Collections.

The greatest threat to potato production world-wide is late blight, caused by the oomycete pathogen Phytophthora infestans. A screen of 126 wild diploid Solanum accessions from the Commonwealth Potato Collection (CPC) with P. infestans isolates belonging to the genotype 13-A2 identified resistances in the species S.