Publications by authors named "J Litch"
Introduction: Selecting an in vitro culture model of the human placenta is challenging due to representation of different trophoblast cell types with distinct biological roles and limited comparative studies that define key characteristics of these models. The aim of this research was to compare the transcriptomes of common in vitro models of the human placenta compared to bulk human placental tissue.
Methods: We performed differential gene expression analysis on publicly available transcriptomic data from 7 in vitro models of the human placenta (HTR-8/SVneo, BeWo, JEG-3, JAR, Primary Trophoblasts, Villous Explants, and Trophoblast Stem Cells) and compared to bulk placental tissue from 2 cohort studies (CANDLE and GAPPS) or individual trophoblast cell types derived from bulk placental tissue.
Studying the human placenta through in vitro cell culture methods is necessary due to limited access and amenability of human placental tissue to certain experimental methods as well as distinct anatomical and physiological differences between animal and human placentas. Selecting an in vitro culture model of the human placenta is challenging due to representation of different trophoblast cell types with distinct biological roles and limited comparative studies that define key characteristics of these models. Therefore, the aim of this research was to create a comprehensive transcriptomic comparison of common in vitro models of the human placenta compared to bulk placental tissue from the CANDLE and GAPPS cohorts (N=1083).
View Article and Find Full Text PDFArticle Synopsis
- This study investigated how maternal copper levels during pregnancy affect the risk of preterm birth (PTB) and the length of gestation.
- In a large group of 10,449 pregnancies from 18 different regions, researchers found that higher maternal copper levels were linked to an increased risk of PTB and shorter pregnancy duration.
- The findings suggest that elevated copper levels may be associated with inflammation and infections, potentially impacting pregnancy outcomes.
We propose adding the category of “at-risk” newborns for babies who are at increased risk of morbidity and/or mortality but do not require special or intensive care or monitoring to promote a 3-tiered newborn care approach in hospitals.
View Article and Find Full Text PDF